Application

epithelial function, tissue repair, cell signaling, lung injury treatment, mechanical and oxidative stress tests, lung lubrication, humidity maintenance, cleaning of respiratory tract, testing of drugs, toxins and carcinogens, respiratory function

Cell Line Origin

Trachea

Components

Basal Medium containing 10% FBS & 10% DMSO

General description

HTEpC provide a useful model system to study all aspects of epithelial function and disease, particularly those related to airway viral infections, as well as tissue repair mechanisms, signaling changes and potential treatments relevant to lung injuries, mechanical and oxidative stress, pulmonary diseases and smoking. HTEpC have been used in a study investigating the role of goblet cell hyperplasia, possibly mediated by the expression of SPDEF, to the pathogenesis of chronic pulmonary diseases (Park, 2007). Characterization: the cells have a characteristic morphology consistent with an epithelial origin and are positive for epithelial cell marker cytokeratin 18.

Preparation Note

1st passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO Can be cultured at least 16 doublings

Subculture Routine

Please refer to the HTEpC Culture Protocol.